fitc-conjugated cd90 antibody  (STEMCELL Technologies Inc)

Journal: International Journal of Molecular Sciences

Article Title: The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres

doi: 10.3390/ijms262412025

Figure Lengend Snippet: Cell and extracellular matrix (ECM) composition of mCSs formed in ULA U-well plates and PDL-coated plates. ( a , b ): Images of mCSs stained by endothelial cell (EC) marker (CD31, red), progenitor cell markers (c-Kit, red; Sca-1, red; Oct4, red), smooth-muscle cell marker (SMA, green), mesenchymal markers (CD105, red; CD90, green; CD73, green), and ECM proteins (COL I, green; FN, green). Nuclei were stained with DAPI. Yellow arrows indicate positive staining. ( c ) Bar plot of a positive staining cell fraction per nuclei within the mCS slice, n = 3, * p < 0.05. ( d ) Bar plot of fluorescence intensity signal normalized per mCS slice area, n = 3, ** p < 0.01. SMA—smooth muscle actin, COL I—collagen type I, FN—fibronectin.

Article Snippet: The EDCs were trypsinized, washed in ice-cold FASC buffer (5% FBS, 0.1% sodium azide in Ca 2+ , Mg 2+ -free DPBS), and incubated with primary antibodies to CD105 (#120404, Biolegend, San Diego, CA, USA), Sca-1 conjugated with PE/Cy7 (E-AB-F1191UH, Elabscience, Wuhan, China), CD90 conjugated with FITC (E-AB-F1283UC, Elabscience), CD31 (#553370; BD, San Diego, CA, USA), and CD45 conjugated with PE (E-AB-F1136UD, Elabscience) or with isotype IgGs for 30 min on ice.

Techniques: Staining, Marker, Fluorescence

Journal: NPJ Precision Oncology

Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer

doi: 10.1038/s41698-025-01040-2

Figure Lengend Snippet: A Flow cytometry was used to detect CD34 + CD90 + ECs in 2 GBC tissues (PE: CD34, FITC: CD90). B Representative multiple immune staining images of CD34 and CD90 in GBC tissues. C Immune staining of CD34 and CD90 on GBC tissue microarray. D Analysis of the correlation between CD34 + or CD34 + CD90 + cell contents and patient survival prognosis and clinical characteristics. OS, overall survival time.

Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an EasySepTM Release Human FITC Positive Selection Kit (STEMCELL Technologies) was used to isolate CD34 + CD90 + endothelial cells.

Techniques: Flow Cytometry, Staining, Microarray

Journal: NPJ Precision Oncology

Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer

doi: 10.1038/s41698-025-01040-2

Figure Lengend Snippet: A Graphic flow chart showing of isolation process for CD34 + CD90 - and CD34 + CD90 + cells from GBC tissues using magnetic bead sorting. B Identify the purity of CD34 + CD90 + ECs before and after sorting by flow cytometry. C Representative images showing the morphology of primary CD34 + CD90 - (upper) and CD34 + CD90 + (lower) ECs. D The mRNA levels of mesenchymal transition-related genes were determined by qRT-PCR, a heatmap showing the relative ratios of CD34 + CD90 + /CD34 + CD90 - in 3 GBC samples. E Representative IF staining images showing the protein levels of CD34, CD90, and p-Smad2/3 in GBC tissue. F GBC-SD cells were co-cultured with isolated CD34 + CD90 + or CD34 + CD90 - cells. Representative images and quantification showing the effects of CD34 + CD90 + or CD34 + CD90 - cells on GBC-SD cell migration and invasion. G Transcriptional levels of mesenchymal transition-related genes MMP-9, FN-1, and TWIST in CD34 + CD90 + and CD34 + CD90 - cells with or without TGF-β pathway inhibitors (ITD, PFD) treatment were determined by qRT-PCR. H GBC-SD cells were co-cultured with isolated CD34 + CD90 + cells with or without MMP-9 antibodies and TGF-β pathway inhibitors (ITD, PFD) treatment. Representative images and quantification showing metastatic cell numbers in each group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, not significant.

Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an EasySepTM Release Human FITC Positive Selection Kit (STEMCELL Technologies) was used to isolate CD34 + CD90 + endothelial cells.

Techniques: Isolation, Flow Cytometry, Quantitative RT-PCR, Staining, Cell Culture, Migration

Journal: NPJ Precision Oncology

Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer

doi: 10.1038/s41698-025-01040-2

Figure Lengend Snippet: A Workflow of in vivo liver metastasis experiment. B In vivo GBC-SD tumor growth ability was assessed using bioluminescence. C Identification of CD34 + CD90 + and CD34 + CD90 - cells in mouse liver tumor tissue by IF staining. D Representative images of mouse livers and H&E staining. E Representative images and quantification showing the IHC staining of MMP-9, FN1, and PDGFD in GBC-SD tumor-bearing mouse liver tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant.

Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an EasySepTM Release Human FITC Positive Selection Kit (STEMCELL Technologies) was used to isolate CD34 + CD90 + endothelial cells.

Techniques: In Vivo, Staining, Immunohistochemistry

Journal: NPJ Precision Oncology

Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer

doi: 10.1038/s41698-025-01040-2

Figure Lengend Snippet: A UMAP plot displaying major cell clusters and EC subclusters from single-cell data of GSE149614 . B Bubble plot showing expression levels of endothelial marker genes for each subset. C Heat maps showing Ro/e of endothelial subsets in non-tumor (N) and HCC tumor (T) tissues. D Bar chart displaying the distribution of each EC subset in non-tumor (N) and tumor (T) tissues. E Bubble plot and heatmap chart displaying the enriched pathways for each EC subset analyzed by GO. F Violin plots showing expression scores for extracellular matrix remodeling and angiogenesis-related genes for each EC subset. G Multiple IHC showing the CD34 + CD90 + ECs in HCC tumor tissue.

Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an EasySepTM Release Human FITC Positive Selection Kit (STEMCELL Technologies) was used to isolate CD34 + CD90 + endothelial cells.

Techniques: Expressing, Marker

Journal: Stem Cell Research & Therapy

Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils

doi: 10.1186/s13287-024-03882-2

Figure Lengend Snippet: Characteristic and functional identification of E-DFSCs. ( A ) Flow chart of experiments in vitro. ( B ) Flow cytometry detection of markers CD34, CD45, CD44, CD90 and CD105 in DFSCs. ( C ) The image of E-DFSCs in the fluorescence microscope. Scale bar: 100 μm. ( D ) The OD values of three kinds of DFSCs proliferation were tested by the CCK-8 kit at 450 nm. ( E ) The three kinds of DFSCs for alizarin red staining of osteogenesis and oil red O staining of adipogenesis after 2 weeks of induction. Scale bar:100 μm. ( F ) The qRT-PCR for osteogenesis relative gene in three kinds of DFSCs with 2 weeks induction. ( G, H ) The images and quantitative analysis of PDLCs migration without DFSCs or with three kinds of DFSCs. Scale bar: 100 μm. ( I, J ) Flow cytometric analysis for CD206 and CD86 surface markers expression on macrophages and calculation of CD206/CD86 value after co-culture without DFSCs or with three kinds of DFSCs. DFSC represents the DFSCs uninfected with any lentivirus, E-DFSC represents the DFSCs infected with lentivirus of CD63-EGFP-Puro overexpression and lentivirus of ACTB-mCherry-NEO overexpression, and C-DFSC represents the DFSCs infected with lentivirus of Puro overexpression and lentivirus of NEO overexpression. The data were analyzed by ordinary one-way ANOVA with multiple comparisons which compare the mean of each group with the mean of every other group and the Bonferroni post hoc test. “*” means statistical significance between any two groups. “#” means statistical significance between the control group with any other group. When three groups were compared in pairs, α*=α/3, * or # p < 0.017; ** or ## p < 0.003; *** or ### p < 0.0003. When four groups were compared in pairs, α*=α/6, * or # p < 0.0083; ** or ## p < 0.0017; *** or ### p < 0.00017. Error bars represent means ± SD

Article Snippet: To evaluate surface markers, cells were incubated with fluorescein isothiocyanate (FITC)-conjugated antibodies against CD44, CD34, CD45, CD90, and CD105 from BD Biosciences (USA), or with phycoerythrin-cyanine7 (PE-Cy7)-conjugated antibodies against CD86 and allophycocyanin (APC)-conjugated antibodies against CD206 from eBioscience(USA) for 30 min at 4 °C.

Techniques: Functional Assay, In Vitro, Flow Cytometry, Fluorescence, Microscopy, CCK-8 Assay, Staining, Quantitative RT-PCR, Migration, Expressing, Co-Culture Assay, Infection, Over Expression, Control